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Peak Splitting in HPLC
#1 · September 29, 2024, 6:15 am
What are common causes of peak splitting in HPLC?
How to resolve it?
#2 · October 3, 2024, 12:05 am
The two common causes of peak splitting in HPLC are a blocked frit and a void in the packing at the head of the column.
- The problem of the peak splitting can be solved by reverse flushing most of the times as it removes the contaminant from the column.
- Washing the column with 90% Organic phase such as Acetonitrile or Methanol can also solved the problem.